Journal: Indiana University journal of undergraduate research

Article Title: Using the Predicted Structure of the Amot Coiled Coil Homology Domain to Understand Lipid Binding

doi: 10.14434/iujur.v4i1.24528

Figure Lengend Snippet: This figure shows the characterization of peptides in Figure 2/Table 1 for their net charge and hydrophobicity using a peptide property calculator (LifeTein).

Article Snippet: This figure shows the characterization of peptides in / for their net charge and hydrophobicity using a peptide property calculator (LifeTein). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Spot Amino Acid Sequence Spot Amino Acid Sequence 0 MPRAQPSSASYQPVP 0 MPRAQPSSASYQPVP 1 AQPSSASYQPVPADP 1 AQPSSASYQPVPADP 2 SSASYQPVPADPFAC 2 SSASYQPVPADPFAC 3 SYQPVPADPFACIVS 3 SYQPVPADPFACIVS 4 PVPADPFACIVSRAQ 4 PVPADPFACIVSRAQ 5 ADPFACIVSRAQQMV 5 ADPFACIVSRAQQMV 6 FACIVSRAQQMVEIL 6 FACIVSRAQQMVEIL 7 IVSRAQQMVEILSDE 7 IVSRAQQMVEILSDE 8 RAQQMVEILSDENRN 8 RAQQMVEILSDENRN 9 QMVEILSDENRNLRQ 9 QMVEILSDENRNLRQ 10 EILSDENRNLRQELE 10 EILSDENRNLRQELE 11 SDENRNLRQELEGCY 11 SDENRNLRQELEGCY 12 NRNLRQELEGCYEKV 12 NRNLRQELEGCYEKV 13 LRQELEGCYEKVARL 13 LRQELEGCYEKVARL 14 ELEGCYEKVARLQKV 14 ELEGCYEKVARLQKV 15 GCYEKVARLQKVETE 15 GCYEKVARLQKVETE 16 EKVARLQKVETEIQR 16 EKVARLQKVETEIQR 17 ARLQKVETEIQRVSE 17 ARLQKVETEIQRVSE 18 QKVETEIQRVSEAYE 18 QKVETEIQRVSEAYE 19 ETEIQRVSEAYENLV 19 ETEIQRVSEAYENLV 20 IQRVSEAYENLVKSS 20 IQRVSEAYENLVKSS 21 VSEAYENLVKSSSKR 21 VSEAYENLVKSSSKR 22 AYENLVKSSSKREAL 22 AYENLVKSSSKREAL 23 NLVKSSSKREALEKA 23 NLVKSSSKREALEKA 24 KSSSKREALEKAMRN 24 KSSSKREALEKAMRN 25 SKREALEKAMRNKLE 25 SKREALEKAMRNKLE 26 EALEKAMRNKLEGEI 26 EALEKAMRNKLEGEI 27 EKAMRNKLEGEIRRM 27 EKAMRNKLEGEIRRM 28 MRNKLEGEIRRMHDF 28 MRNKLEGEIRRMHDF 29 KLEGEIRRMHDFNRD 29 KLEGEIRRMHDFNRD 30 GEIRRMHDFNRDLRE 30 GEIRRMHDFNRDLRE 31 RRMHDFNRDLRERLE 31 RRMHDFNRDLRERLE 32 HDFNRDLRERLETAN 32 HDFNRDLRERLETAN 33 NRDLRERLETANKQL 33 NRDLRERLETANKQL 34 LRERLETANKQLAEK 34 LRERLETANKQLAEK 35 RLETANKQLAEKEYE 35 RLETANKQLAEKEYE 36 TANKQLAEKEYEGSE 36 TANKQLAEKEYEGSE 37 KQLAEKEYEGSEDTR 37 KQLAEKEYEGSEDTR 38 AEKEYEGSEDTRKTI 38 AEKEYEGSEDTRKTI 39 EYEGSEDTRKTISQL Open in a separate window Peptide Spot Blot Amino Acid Sequences table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Spot % Intensity in SPOTs Assay Targeted Amot80 Residues Net Charge at pH 7 Basic residues Hydrophobicity at pH 6.8 Tyrosine Residues 5 44.0 16−30 −0.09 R25 30.27 14 42.6 43−57 −0.09 K50, R53, K56 22.8 Y48 21 62.9 64−78 1 K73, K77, R78 11.53 Y68 23 53.9 70−84 2 K73, K77, R78, K83 10.47 42 80.8 127−141 3 K127, K135, K137, R141 11 55 55.8 166−180 2 R167, K176, K180 19.53 69 88.8 208−222 2.02 K213, R214, 220, R222 8.67 76 62.1 229−243 1.91 R231, R235 4 Open in a separate window Characterization of Specific Peptides from Speciftcity of peptide affinity.

Techniques: